What is the parasite classification of blastocystis hominis?
Blastocystis Hominis is a protozoan intestinal parasite belonging to the Blastocystis genus of Stramenopiles – a vast array of organisms including brown algae, water molds, and diatoms. It has a widespread geographic distribution and is found in countries of all income levels across the world.
What are the different forms of blastocystis hominis?
Four common forms of Blastocystis hominis. Clockwise from top left: vacuolar, granular, amoeboid, and cyst forms.
How blastocystis is diagnosed?
A blood test that can detect blastocystis is available but not commonly used. However, your doctor might order blood tests to look for other causes of your signs and symptoms.
What is the best screening method for the identification of blastocystis hominis?
Among the three, PCR is considered the gold standard test for detection of Blastocystis, as it helps to authenticate the sensitivity, specificity, and kappa agreement obtained by the other two tests.
How common is blastocystis hominis?
Blastocystis hominis (Brumpt, 1912) is a unicellular protozoan found in the large intestine in humans. It is currently the predominant parasite found in human stool samples, with a higher prevalence in developing countries (50–60%) than developed countries (about 10% or less).
What size is blastocystis hominis?
Unicellular, it is 5-30 microns in diameter, with the usual range being 8-10 microns. Blastocystis is usually spherical, oval, or ellipsoidal, with usually one, but sometimes two to four nuclei located in the rim of the cytoplasm.
What is the infective stage of blastocystis hominis?
The cyst form (3–5 µm) is postulated to be an infectious stage, but not confirmed. The predominant form found in human stool specimens is referred to as the vacuolar (or central body) form and is of variable size (5–40 µm, occasionally much larger). Replication appears to occur via binary fission.
Does blastocystis hominis cause weight gain?
hominis (Blasto-Ag). Group (b) exhibited the highest level of weight gain. Group (d) had higher levels of parasite cyst count in stools, serum IgE, oxidized protein and lipid compared to the group (c).
Can Blastocystis hominis make you sick?
What are the symptoms? Many people with Blastocystis hominis in their stools have no symptoms at all. However, in those that do report symptoms, the most common ones are diarrhoea, abdominal pain and vomiting.
Can blastocystis hominis be cured?
Blastocystis sp. is a parasite which does not need to be cured with the antibiotics that cause side effects, such as metronidazole (MTZ).
What are the different types of Blastocystis hominis?
Morphology: Culture studies of Blastocystis Hominishave identified several main forms of the organism as well as a recently confirmed cyst stage.  -Vacuolar and granular forms: These consist of a spherical cell containing a large single vacuole. Granular cells contain many small granules in the cytoplasm or the central vacuole.
How does the vacuolar stage of Blastocystis reproduce?
The CDC approximation indicates the following, however. It is proposed that within the gut, vacuolar stages reproduce by mitosis, occasionally forming multi-vacuolar or ameboid forms. The ameboid form replicates further through mitosis, eventually undergoing schizogony before exiting the host as a thick-walled cyst.
How is Blastocystis sp.detected in human stool?
The vacuoles stain variably from red to blue with trichrome; the staining facilitates detection and identification of the organism (e.g., differentiation from fecal debris). Other morphologic forms occasionally are noted in human stool specimens. Figure A: Blastocystis sp. in an unstained wet mount.
How is Blastocystis sp.formed in a wet mount?
Figure A: Blastocystis sp. in a wet mount under differential interference contrast (DIC) microscopy. Figure B: Blastocystis sp. in a wet mount under differential interference contrast (DIC) microscopy. Blastocystis sp. vacuolar forms in wet mounts stained with iodine.