What is an EST database?
The expressed sequence tag (EST) databases contain an increasingly large part of the transcriptome of many species. For this reason, these databases are probably the most abundant source of new coding sequences available today.
What is EST technique?
In software engineering, test design technique is a procedure for determining test conditions, test cases and test data during software testing. Test design techniques always include test selection criteria determining when to stop designing more test cases.
What is EST in genomics?
Abstract. Expressed sequence tags (ESTs) are fragments of mRNA sequences derived through single sequencing reactions performed on randomly selected clones from cDNA libraries. To date, over 45 million ESTs have been generated from over 1400 different species of eukaryotes.
What is an EST and why would you expect to find such results for a particular gene?
EST sequences contain at least partial sequences of most mRNAs present in the various tissues used for library construction. Therefore, they have been used intensively as a source of information for the discovery of new genes whose function can be tentatively deduced from their sequence, and experimentally verified.
What is EST evidence?
In genetics, an expressed sequence tag (EST) is a short sub-sequence of a cDNA sequence. ESTs may be used to identify gene transcripts, and were instrumental in gene discovery and in gene-sequence determination. An EST results from one-shot sequencing of a cloned cDNA.
What is EST analysis?
ESTs are unique DNA sequences generated by sequencing cDNA clones from cDNA libraries. In the Sanger sequencing era, EST sequencing served as an important approach in identification and analysis of gene transcripts and expression (Adams et al., 1991).
How do you generate EST?
ESTs are generated by sequencing cDNA, which itself is synthesized from the mRNA molecules in a cell. The mRNA in a cell are copies of the genes that are being expressed.