Do endothelial cells express selectin?
E-Selectin (CD62E) is a 115 kDa glycoprotein, only expressed on endothelial cells after activation by interleukin 1 (IL-1), tumor necrosis factor α (TNFα) or bacterial lipopolysaccharides.
Where is E-selectin found?
Letter: E-selectin was not found in amniotic fluid but was found in cord blood. CD40/CD40L interaction induces E-selectin dependent adhesion of leukocytes to human endothelial cells and reduces endothelial cell migration by inhibiting the Akt/eNOS signaling pathway.
What is soluble E-selectin?
Soluble E-selectin (sE-selectin) is shed into the circulation from the activated endothelium. We investigated the effect of sE-selectin on shear-resistant adhesion and migration of metastatic breast cancer cells and leukocytes.
What are selectin ligands?
The selectins (cluster of differentiation 62 or CD62) are a family of cell adhesion molecules (or CAMs). Selectins bind to sugar moieties and so are considered to be a type of lectin, cell adhesion proteins that bind sugar polymers.
What does P-selectin bind to?
The primary ligand for P-selectin is P-selectin glycoprotein ligand-1 (PSGL-1) which is expressed on almost all leukocytes, although P-selectin also binds to heparan sulfate and fucoidans.
What does L-selectin bind?
Similar to P-selectin, L-selectin also binds to sulfatides and sulfated polysaccharides such as fucoidan and heparin. A group of O-glycosylated mucins decorated with sialic acid, termed sialomucins, represents the protein ligands for L-selectin. They are GlyCAM-1, CD34 and MAdCAM-1.
What does E-selectin stand for?
E-selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), is a selectin cell adhesion molecule expressed only on endothelial cells activated by cytokines.
Are selectins inflammatory mediators?
“Basal” expression of E-selectin is usually low and several inflammatory mediators, including tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), CD40 ligand, and lipopolysaccharide (LPS) stimulate expression by activating transcription.
What does L selectin bind?
Is selectin and integrin?
Selectins and some of their counter-receptors function also as signal-transducing receptors, significantly contributing to leukocyte and endothelial cell activation. Integrins represent a large family of adhesion receptors that are widely expressed and mainly interact with extracellular matrix components.
What stimulates P-selectin?
Thrombin is one trigger which can stimulate endothelial-cell release of P-selectin and recent studies suggest an additional Ca2+-independent pathway involved in the release of P-selectin. The P-selectin then promotes platelet aggregation through platelet-fibrin and platelet-platelet binding.
Is P-selectin and integrin?
P-selectin and PSGL-1 constitute the main receptor–ligand pair that mediates physical interactions between platelets and leukocytes. Activation of the leukocyte β1 and β2 integrins involves functional upregulation and activation-dependent conformational changes necessary for the integrins to recognize their ligands.
How is E-selectin upregulated in endothelial cells?
In unstimulated endothelial cells, E-selectin is undetectable. In vivo studies in mice show that E-selectin is upregulated after two hours of TNF-α stimulation, suggesting regulation at the transcriptional level. Other stimuli involved in upregulation of selectins are interleukin-lβ (IL-1β) and lipopolysaccharide (LPS).
What is the role of E-selectin in leukocyte rolling?
E-selectin is an adhesion receptor involved in slowing down leukocyte rolling and its expression is restricted to endothelial cells. In unstimulated endothelial cells, E-selectin is undetectable. In vivo studies in mice show that E-selectin is upregulated after two hours of TNF-α stimulation, suggesting regulation at the transcriptional level.
What kind of diseases are associated with E-selectin?
E-selectin is upregulated in proliferating endothelial cells in culture , and elevated soluble E-selectin has been associated with vasculoproliferative disorders, such as rheumatoid arthritis and various cancers [163–166].
How is E-selectin different from other selectins?
Unlike PSGL-1 binding to P-selectin, its binding to E-selectin does not require core2GlcNAcT-I , or sulfotransferases. Like the other selectins, E-selectin supports leukocyte rolling on inflamed endothelial cells in vitro  and in vivo .