What is anti GFP antibody?
The target antigen refers to green fluorescent protein (GFP), commonly used as an epitope tag for recombinant proteins for detection. GFP is a 27 kilodalton protein that can be detected in cells both in vivo and in vitro for studies in gene expression.
What is a GFP booster?
ChromoTek offers the GFP-Booster which are GFP-binding proteins coupled to a fluorescent dye (from ATTO-TEC). The GFP-Booster can be used to increase, stabilize and reactivate fluorescent protein signals for immunofluorescence (IF) staining.
Do GFP antibodies recognize EGFP?
GFP antibody (ab6556) is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP and EGFP.
Can GFP antibody detect CFP?
Q: What GFP variants will this antibody recognize? A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.
Does mCherry antibody recognize RFP?
The anti-mCherry monoclonal antibody recognizes RFP (DsRed) and its variants including mCherry, plum, and probably other RFP-derived fluorescent proteins.
How are antibodies used in immunofluorescence?
Primary, or direct, immunofluorescence uses a single antibody that is chemically linked to a fluorophore. The antibody recognizes the target molecule and binds to it, and the fluorophore it carries can be detected via microscopy.
Why are fluorescent reagents kept in the dark?
Store fluorescent reagents appropriately Clark emphasizes that “fluorescent species must be stored carefully at the recommended temperature and kept in the dark at all times to protect their spectral integrity.
What is mCherry antibody?
mCherry is a fluorescent protein derived from Discosoma sp. Mushroom corals. Our antibodies for mCherry can be used to detect native and denatured forms of mCherry or mCherry fusion proteins in various applications.