What does Neisseria gonorrhoeae need to grow?
A fastidious organism, N. gonorrhoeae requires enriched media in a CO2 atmosphere at 35 degrees to 37 degrees C for growth. Long believed to be an obligate aerobe, the gonococcus is capable of anaerobic growth when provided with a suitable electron acceptor.
Which medium is selective for growth of Neisseria?
GC-Lect, a new selective medium for the isolation of Neisseria gonorrhoeae which contains five antimicrobial agents, was evaluated with stock cultures and with 500 clinical specimens.
What culture media is used for Neisseria gonorrhoeae?
Neisseria gonorrhoeae is one of the causatives of sexually transmitted diseases, and it is a fastidious organism. This organism is typically cultured using an agar medium such as chocolate agar plate (GCII agar base with 1% IsoVitaleX [BBL] and purified hemoglobin).
Can Neisseria gonorrhoeae grow on nutrient agar?
Ability to grow on nutrient agar is one of the criteria to differentiate commensal Neisseria spp. and M. catarrhalis from Neisseria gonorrhoeae or Neisseria meningitidis (1, 3). There are now several commercial media of nutrient agar, the formulas of which are somewhat different among the manufactur- ers.
Where is Neisseria found?
The Gram-negative bacteria genus Neisseria includes both pathogenic and commensal species that are found primarily in the upper respiratory tract of humans and animals.
How long does it take to grow Neisseria gonorrhoeae?
Blood agar and chocolate medium (blood heated at 176–194°F/80–90°C) are suitable growth media. Bacterial colonies usually appear after 24–48 hours of growth. Colonies of N. gonorrhoeae are 0.5–1 mm in size.
How do you confirm Neisseria gonorrhoeae?
A Definitive diagnosis of gonorrhea requires: isolation of N. gonorrhoeae from sites of exposure (e.g., urethra, endocervix, throat, rectum) by culture (usually a selective medium) and demonstrating typical colonial morphology, positive oxidase reaction, and typical gram-negative morphology and.
Does gonorrhea grow on agar?
Neisseria gonorrhoeae is the causative agent of gonococcal infections. Gonococci are not able to grow on common blood agar. Most Neisseria strains have complex growth requirements. Some strains may be exquisitely sensitive to fatty acids, necessitating the incorporation of soluble starch in the growth media.
Why does gonorrhea not grow on blood agar?
Neisseria gonorrhoeae are the most fastidious of the Neisseria species, require complex growth media and are highly susceptible to toxic substnces (e.g., fatty acids). Gonococci are not able to grow on common blood agar.
How do you identify Neisseria?
N. meningitidis can be identified using Kovac’s oxidase test and carbohydrate utilization. If the oxidase test is positive, carbohydrate utilization testing should be performed. If the carbohydrate utilization test indicates that the isolate may be N.
How long does it take for Neisseria bacteria to grow?
Blood agar and chocolate medium (blood heated at 176–194°F/80–90°C) are suitable growth media. Bacterial colonies usually appear after 24–48 hours of growth. Colonies of N. gonorrhoeae are 0.5–1 mm in size. Colonies of N. meningitidis are usually larger (1–2 mm) and flatter.
What kind of atmosphere does Neisseria spp grow in?
Neisseria spp. grow best aerobically in an atmosphere containing 5–10% carbon dioxide at a temperature of 89.6–98.6°F (32–37°C) and a pH of 7–7.5. Cell size ranges from 0.6 to 1.5 mm depending upon the species source of the isolate and the age of the culture. Neisseria spp. are fastidious.
What kind of agar plate does Neisseria grow on?
2(or in a candle-jar). It can grow on both a blood agar plate (BAP) and a chocolate agar plate (CAP) . Colonies of N. meningitidis are grey and unpigmented on a BAP and appear round, smooth, moist, glistening, and convex, with a clearly defined edge.N. meningitidisappear as large, colorless-to-grey, opaque colonies on a CAP.
What kind of oxygen does Neisseria meningitidis need?
We demonstrate here that although N. meningitidis fails to grow under strictly anaerobic conditions, under oxygen limitation the bacterium expresses a denitrification pathway (reduction of nitrite to nitrous oxide via nitric oxide) and that this pathway supplements growth.