What are Neb enzymes?

What are Neb enzymes?

NEB has developed a line of High-Fidelity (HF®) restriction enzymes. These engineered enzymes have the same specificity as the native enzyme, with the added benefit of reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart buffer.

What is enzyme DdeI?

DdeI is a DNA restriction enzyme used in molecular biology research that cleaves the recognition sequence 5′-C/TNAG-3′, generating 5′-cohesive termini.

What is the products of restriction enzymes?

Restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms.

What is in restriction enzyme buffer?

MULTI-CORE™ Buffer (1X) = 25mM Tris-acetate (pH 7.5 at 37°C), 100mM potassium acetate, 10mM magnesium acetate, 1mM DTT. Notes: For each 10°C rise in temperature between 0°C and 25°C, the pH of Tris buffers decreases 0.31 pH units.

Why would restriction enzymes not work?

There can be several different reasons why your restriction enzyme does not cut the DNA as reviewed in this video. The preparation of DNA to be cleaved should be free of contaminants such as phenol, chloroform, alcohol, EDTA, detergents, or excessive salts, all of which can interfere with restriction enzyme activity.

How to find a restriction enzyme in Neb?

Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang. Enzyme Finder | NEB Enzyme Finder version {{appVersion}}

Where does the Apali gene come from in E coli?

Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality. An E. coli strain that carries the ApaLI gene from Acetobacter pasteurianus (ATCC 12875).

What is the half life of Apai at 37°C?

Incubation at 37°C results in 100% activity, however, the half-life of ApaI at 37°C is only 30 minutes. ApaI is inhibited by salt concentrations above 50 mM. Blocked by overlapping dcm methylation and overlapping CpG methylation. Why Choose Recombinant Enzymes? Is ApaI activity sensitive to dam, dcm or mammalian CpG methylation?

What are the 4 base sequences of Neb enzymes?

All Enzymes NEB Enzymes Type IIS 4-base Sequences 6-base Sequences Long Sequences Thermophilic Time-Saver??? Qualified High-Fidelity (HF??) Recombinant EpiMark??