What is F4 80 a marker for?

What is F4 80 a marker for?

The F4/80 molecule was established as a unique marker of murine macrophages when a monoclonal antibody was found to recognize an antigen exclusively expressed by these cells.

Is F4 80 expressed in human macrophages?

F4/80: Expression F4/80 is expressed at high levels on the surface of various macrophages: kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria, and langerhans cells in the skin.

Do T cells express F4 80?

The role of F4/80 in the generation of T reg cells for peripheral tolerance in vitro. It is known that inoculation of antigen via the a.c. results in the appearance of tolerance-inducing F4/80+ cells in the peripheral blood within 3 d (20, 31).

Are neutrophils F4 80?

Now take into account that there are other cell in the tissues: neutrophils, DCs, eosinophils, mast cells – they can overlap with macrophages and obscure them. Monocytes are F4/80low and most of the monocyte-derived macrophages are F4/80low. Eosinophils express F4/80 as well (though less than macrophages).

Is F4 80 expressed on monocytes?

F4/80 has generally been considered to be a marker specific for both circulating monocytes and tissue-residing monocytes and macrophages, yet its expression is not limited to these cell populations (6,7).

What are F4 80 cells?

F4/80 antigen is a mature mouse cell surface glycoprotein expressed at high levels on various macrophages including: Kupffer cells, splenic red pulp macrophages, microglia, gut lamina propria, and Langerhans cells in the skin.

What is Ly6C high?

Monocytes expressing high levels of Ly6C (Ly6Chi monocytes) have proinflammatory and antimicrobial functions and express high levels of C-C chemokine receptor 2 (CCR2) and low levels of CX3C chemokine receptor 1 (CX3CR1; CCR2hiCX3CR1low).

Do T cells express Ly6C?

Ly6C is a murine cell-surface antigen expressed by plasma cells, subsets of myeloid cells and many T cells, including memory T cells.

How long does a macrophage live?

The lifespan varies with the “lesion” and “tissue”. In general, tissue resident macrophages are long-lived cells – from more than 3-days to weeks. Again, the life span varies with species. Unlike neutrophils, which are short-lived, macrophages can live for months to years.

What does ly6c stand for?

Official Symbol Ly6c1provided by MGI Official Full Name lymphocyte antigen 6 complex, locus C1provided by MGI Primary source MGI:MGI:96882 See related Ensembl:ENSMUSG00000079018 Gene type protein coding RefSeq status VALIDATED Organism Mus musculus Lineage Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; …

What is the lifespan of platelets?

Platelets (thrombocytes) The lifespan of platelets is about 9 to 12 days.

Which is the mouse macrophage marker F4 / 80?

F4/80 antibody clone CI:A3-1 (MCA497), is a well-characterized and extensively referenced mouse macrophage marker. Also known as Ly71 and EMR1, the F4/80 antigen is part of the EGF-TM7 family, it is a 160 kDa glycoprotein that shares 68% amino acid identity with human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1).

What kind of antibody recognises the F4 / 80 antigen?

Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.

Which is the best F4 / 80 antibody for microglia?

F4/80 antibody (MCA497, clone CI:A3-1) is the original and the best: High specificity – shown to be highly targeted for all mouse macrophages. Added versatility for microglial cells – unlike many other macrophage markers F4/80 is present on microglia which makes it perfect for detecting and isolating these cells in neurological research.

Where is F4 / 80 expressed in the body?

F4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1).